Reaction: (S)-lactate + NAD+ = pyruvate + NADH + H+
Other name(s): lactic acid dehydrogenase; L(+)-nLDH; L-(+)-lactate dehydrogenase; L-lactic dehydrogenase; L-lactic acid dehydrogenase; lactate dehydrogenase; lactate dehydrogenase NAD-dependent; lactic dehydrogenase; NAD-lactate dehydrogenase
Systematic name: (S)-lactate:NAD+ oxidoreductase
Comments: Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9001-60-9
References:
1. Dennis, D. and Kaplan, N.O. D and L-lactic acid dehydrogenase in Lactobacillus plantarum. J. Biol. Chem. 235 (1960) 810-818.
2. Everse, J. and Kaplan, N.O. Lactate dehydrogenases: structure and function. Adv. Enzymol. Relat. Subj. Biochem. 37 (1973) 61-133. [PMID: 4146647]
3. Holbrook, J.J., Liljas, A., Steindel, S.J. and Rossmann, M.G. Lactate dehydrogenase. In: Boyer, P.D. (Ed.), The Enzymes, 3rd ed., vol. 11, Academic Press, New York, 1975, p. 191-292.
4. Schär, H.-P. and Zuber, H. Structure and function of L-lactate dehydrogenases from thermophilic and mesophilic bacteria. I) Isolation and characterization of lactate dehydrogenases from thermophilic and mesophilic bacilli. Hoppe-Seyler's Z. Physiol. Chem. 360 (1979) 795-807. [PMID: 114469]