IUBMB Enzyme Nomenclature


Accepted name: D-2-hydroxyacid dehydrogenase (NADP+)

Reaction: an (R)-2-hydroxycarboxylate + NADP+ = a 2-oxocarboxylate + NADPH + H+

For diagram of reaction click here

Other name(s): ddh (gene name)

Systematic name: (R)-2-hydroxycarboxylate:NADP+ oxidoreductase

Comments: This enzyme, characterized from the halophilic archaeon Haloferax mediterranei and the mold Aspergillus oryzae, catalyses a stereospecific reduction of 2-oxocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC, (D)-2-hydroxyacid dehydrogenase (NAD+).

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 81210-65-3


1. Domenech, J. and Ferrer, J. A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression. Biochim. Biophys. Acta 1760 (2006) 1667–1674. [PMID: 17049749]

2. Shimizu, M., Yamamoto, T., Okabe, N., Sakai, K., Koide, E., Miyachi, Y., Kurimoto, M., Mochizuki, M., Yoshino-Yasuda, S., Mitsui, S., Ito, A., Murano, H., Takaya, N. and Kato, M. Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate. Appl. Microbiol. Biotechnol. (2015) . [PMID: 26615399]

[EC created 2002]

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