Reaction: 2-(glutathione-S-yl)-2-methylbut-3-en-1-ol + 2 NAD+ + H2O = 2-(glutathione-S-yl)-2-methylbut-3-enoate + 2 NADH + 2 H+ (overall reaction)
(1a) 2-(glutathione-S-yl)-2-methylbut-3-en-1-ol + NAD+ = 2-(glutathione-S-yl)-2-methylbut-3-enal + NADH + H+
(1b) 2-(glutathione-S-yl)-2-methylbut-3-enal + NAD+ + H2O = 2-(glutathione-S-yl)-2-methylbut-3-enoate + NADH + H+
Other name(s): isoH (gene name); 4-hydroxy-3-glutathionyl-3-methylbut-1-ene dehydrogenase
Systematic name: 2-(glutathione-S-yl)-2-methylbut-3-en-1-ol:NAD+ oxidoreductase
Comments: The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. van Hylckama Vlieg, J.E., Kingma, J., Kruizinga, W. and Janssen, D.B. Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45. J. Bacteriol. 181 (1999) 2094-2101. [PMID: 10094686]