Reaction: (1) (S)-malate + NADP+ = pyruvate + CO2 + NADPH
(2) oxaloacetate = pyruvate + CO2
Other name(s): 'malic' enzyme (ambiguous); pyruvic-malic carboxylase (ambiguous); malate dehydrogenase (decarboxylating, NADP+); NADP+-linked decarboxylating malic enzyme; NADP+-malic enzyme; NADP+-specific malic enzyme; NADP-specific malate dehydrogenase; malate dehydrogenase (NADP+, decarboxylating); L-malate:NADP+ oxidoreductase
Systematic name: (S)-malate:NADP+ oxidoreductase (oxaloacetate-decarboxylating)
Comments: The enzyme catalyses the oxidative decarboxylation of (S)-malate in the presence of NADP+ and divalent metal ions, and the decarboxylation of oxaloacetate. cf. EC 18.104.22.168, malate dehydrogenase (oxaloacetate-decarboxylating), and EC 22.214.171.124, malate dehydrogenase (decarboxylating).
Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9028-47-1
1. Harary, I., Korey, S.R. and Ochoa, S. Biosynthesis of dicarboxylic acids by carbon dioxide fixation. VII. Equilibrium of "malic" enzyme reaction. J. Biol. Chem. 203 (1953) 595-604.
2. Ochoa, S., Mehler, A.H. and Kornberg, A. Biosynthesis of dicarboxylic acids by carbon dioxide fixation. I. Isolation and properties of an enzyme from pigeon liver catalyzing the reversible oxidative decarboxylation of l-malic acid. J. Biol. Chem. 174 (1948) 979-1000.
3. Rutter, W.J. and Lardy, H.A. Purification and properties of pigeon liver malic enzyme. J. Biol. Chem. 233 (1958) 374-382.
4. Stickland, R.G. Some properties of the malic enzyme of pigeon liver. 1. Conversion of malate into pyruvate. Biochem. J. 73 (1959) 646-654.
5. Stickland, R.G. Some properties of the malic enzyme of pigeon liver. 2. Synthesis of malate from pyruvate. Biochem. J. 73 (1959) 654-659.
6. Walker, D.A. Physiological studies on acid metabolism. 7. Malic enzyme from Kalanchoë crenata: effects of carbon dioxide concentration. Biochem. J. 74 (1960) 216-223.