Reaction: (1) a ditrans,polycis-polyprenol + NAD(P)+ = a ditrans,polycis-polyprenal + NAD(P)H + H+
(2) a dolichol + NAD(P)+ = a dolichal + NAD(P)H + H+
Other name(s): dolichal reductase; DHRSX (gene name)
Systematic name: ditrans,polycis-polyprenol:NAD(P)+ oxidoreductase
Comments: The enzyme, present in all eukaryotes, catalyses the dehydrogenation of ditrans,polycis-polyprenol and the reduction of dolichal as part of the pathway that converts ditrans,polycis-polyprenol to dolichol (cf. EC 1.3.1.94, polyprenal reductase). The enzyme works equally well with NAD+ and NADP+, but due to the cellular ratios of NAD+/NADH and NADP+/NADPH, the enzyme catalyses the oxidation of of polyprenol using NAD+ as the electron acceptor, and the reduction of dolichal using NADPH as the electron donor.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Wilson, M.P., Kentache, T., Althoff, C.R., Schulz, C., de Bettignies, G., Mateu Cabrera, G., Cimbalistiene, L., Burnyte, B., Yoon, G., Costain, G., Vuillaumier-Barrot, S., Cheillan, D., Rymen, D., Rychtarova, L., Hansikova, H., Bury, M., Dewulf, J.P., Caligiore, F., Jaeken, J., Cantagrel, V., Van Schaftingen, E., Matthijs, G., Foulquier, F. and Bommer, G.T. A pseudoautosomal glycosylation disorder prompts the revision of dolichol biosynthesis. Cell (2024) . [PMID: 38821050]