Reaction: 20β-dihydrocortisol + NAD+ = cortisol + NADH + H+
Other name(s): 20β-HSDH; desE (gene name)
Systematic name: 20β-dihydrocortisol:NAD+ 20-oxidoreductase
Comments: The enzyme, characterized from several gut bacteria, is specific for NADH and is much more active in the reductive direction. cf. the eukaryotic enzyme EC 1.1.1.53, 3α(or 20β)-hydroxysteroid dehydrogenase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Devendran, S., Mendez-Garcia, C. and Ridlon, J.M. Identification and characterization of a 20β-HSDH from the anaerobic gut bacterium Butyricicoccus desmolans ATCC 43058. J. Lipid Res. 58 (2017) 916-925. [PMID: 28314858]
2. Doden, H.L., Pollet, R.M., Mythen, S.M., Wawrzak, Z., Devendran, S., Cann, I., Koropatkin, N.M. and Ridlon, J.M. Structural and biochemical characterization of 20β-hydroxysteroid dehydrogenase from Bifidobacterium adolescentis strain L2-32. J. Biol. Chem. 294 (2019) 12040-12053. [PMID: 31209107]