Reaction: a primary alcohol + O2 = an aldehyde + H2O2
Other name(s): ethanol oxidase
Systematic name: alcohol:oxygen oxidoreductase
Comments: The enzymes from the fungi Candida methanosorbosa and several Basidiomycetes species contain an FAD cofactor [1,3]. The enzyme from the phytopathogenic fungi Colletotrichum graminicola and Colletotrichum gloeosporioides utilize a mononuclear copper-radical mechanism [4]. The enzyme acts on primary alcohols and unsaturated alcohols, and has much lower activity with branched-chain and secondary alcohols.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9073-63-6
References:
1. Janssen, F.W. and Ruelius, H.W. Alcohol oxidase, a flavoprotein from several Basidiomycetes species. Crystallization by fractional precipitation with polyethylene glycol. Biochim. Biophys. Acta 151 (1968) 330-342. [PMID: 5636370]
2. Nishida, A., Ishihara, T. and Hiroi, T. [Studies on enzymes related to lignan biodegradation.] Baiomasu Henkan Keikaku Kenkyu Hokoku (1987) 38-59. (in Japanese)
3. Suye, S. Purification and properties of alcohol oxidase from Candida methanosorbosa M-2003. Curr. Microbiol. 34 (1997) 374-377. [PMID: 9142745]
4. Yin, D.T., Urresti, S., Lafond, M., Johnston, E.M., Derikvand, F., Ciano, L., Berrin, J.G., Henrissat, B., Walton, P.H., Davies, G.J. and Brumer, H. Structure-function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family. Nat Commun 6 (2015) 10197. [PMID: 26680532]