Reaction: (1) N-acetyl-D-glucosamine + O2 + H2O = N-acetyl-D-glucosaminate + H2O2 (overall reaction)
(1a) N-acetyl-D-glucosamine + O2 = N-acetyl-D-glucosamino-1,5-lactone + H2O2
(1b) N-acetyl-D-glucosamino-1,5-lactone + H2O = N-acetyl-D-glucosaminate (spontaneous)
(2) N-acetyl-D-galactosamine + O2 + H2O = N-acetyl-D-galacotsaminate + H2O2 (overall reaction)
(2a) N-acetyl-D-galactosamine + O2 = N-acetyl-D-galactosamino-1,5-lactone + H2O2
(2b) N-acetyl-D-galactosamino-1,5-lactone + H2O = N-acetyl-D-galactosaminate (spontaneous)
Other name(s): N-acyl-D-hexosamine oxidase; N-acyl-β-D-hexosamine:oxygen 1-oxidoreductase
Systematic name: N-acyl-D-hexosamine:oxygen 1-oxidoreductase
Comments: The enzyme, found in bacteria, also acts more slowly on N-acetyl-D-mannosamine.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 121479-58-1
1. Horiuchi, T. Purification and properties of N-acyl-D-hexosamine oxidase from Pseudomonas sp. 15-1. Agric. Biol. Chem. 53 (1989) 361-368.
2. Rembeza, E., Boverio, A., Fraaije, M.W. and Engqvist, M.K.M. Discovery of two novel oxidases using a high-throughput activity screen. ChemBioChem (2021) . [PMID: 34709726]