Reaction: carminate + O2 = 3'-dehydrocarminate + H2O2
For diagram of reaction click here.
Glossary: carminate = 7-carboxy-4,6-dihydroxy-8-methyl-9,10-dioxo-2-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]anthracene-1,3-diolate
Other name(s): carA (gene name)
Systematic name: carminate:oxygen 3'-oxidoreductase (H2O2-forming)
Comments: A flavoprotein (FAD). This bacterial enzyme participates in degradation of certain C-glucosides by catalysing the oxidation of the hydroxyl group at position 3 of the glycose moiety. The enzyme was found active with assorted C-glycosides, such as carminate, mangiferin, and C6-glycosylated flavonoids, but not with D-glucose or C8-glycosylated flavonoids.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Kumano, T., Hori, S., Watanabe, S., Terashita, Y., Yu, H.Y., Hashimoto, Y., Senda, T., Senda, M. and Kobayashi, M. FAD-dependent C-glycoside-metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis. Proc. Natl. Acad. Sci. USA 118 (2021) . [PMID: 34583991]