Reaction: (1) 4-vinylguaiacol + O2 = vanillin + formaldehyde
(2) 4-vinylphenol + O2 = 4-hydroxybenzaldehyde + formaldehyde
Glossary: 4-vinylguaiacol = 2-methoxy-4-vinylphenol
Other name(s): Ado; aromatic dioxygenase
Systematic name: 4-vinylguaiacol:oxygen oxidoreductase
Comments: The enzyme was isolated from the bacterium Caulobacter segnis and the fungus Thermothelomyces thermophilus. It is used as an economic option in lignin degradation since it does not require a cofactor.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Furuya, T., Miura, M. and Kino, K. A coenzyme-independent decarboxylase/oxygenase cascade for the efficient synthesis of vanillin. Chembiochem 15 (2014) 2248-2254. [PMID: 25164030]
2. Ni, J., Wu, Y.T., Tao, F., Peng, Y. and Xu, P. A coenzyme-free biocatalyst for the value-added utilization of lignin-derived aromatics. J. Am. Chem. Soc. 140 (2018) 16001-16005. [PMID: 30376327]