Reaction: 2'-deoxyuridine + 2-oxoglutarate + O2 = uridine + succinate + CO2
Other name(s): deoxyuridine 2'-dioxygenase; deoxyuridine 2'-hydroxylase; pyrimidine deoxyribonucleoside 2'-hydroxylase; thymidine 2'-dioxygenase; thymidine 2'-hydroxylase; thymidine 2-oxoglutarate dioxygenase; thymidine dioxygenase
Systematic name: 2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (2'-hydroxylating)
Comments: Requires iron(II) and ascorbate. Also acts on thymidine. cf. EC 1.14.11.10, pyrimidine-deoxynucleoside 1'-dioxygenase.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 9076-89-5
References:
1. Bankel, L., Lindstedt, G. and Lindstedt, S. Thymidine 2'-hydroxylation in Neurospora crassa. J. Biol. Chem. 247 (1972) 6128-6134. [PMID: 4265566]
2. Stubbe, J. Identification of two α-ketoglutarate-dependent dioxygenases in extracts of Rhodotorula glutinis catalyzing deoxyuridine hydroxylation. J. Biol. Chem. 260 (1985) 9972-9975. [PMID: 4040518]
3. Warn-Cramer, B.J., Macrander, L.A. and Abbott, M.T. Markedly different ascorbate dependencies of the sequential α-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis. J. Biol. Chem. 258 (1983) 10551-10557. [PMID: 6684117]