Reaction: L-enduracididine + 2-oxoglutarate + O2 = (3S)-3-hydroxy-L-enduracididine + succinate + CO2
Glossary: L-enduracididine = 3-[(4R)-2-iminoimidazolidin-4-yl]-L-alanine = 2-amino-3-[(2S)-iminoimidazolin-4-yl]propanoic acid
(3S)-3-hydroxy-L-enduracididine = (2S,3R)-2-amino-3-hydroxy-3-[(S)-2-iminoimidazolidin-4-yl]propanoic acid = (3R)-3-[(4S)-2-iminoimidazolidin-4-yl]-L-serine
Other name(s): MppO; L-enduracididine,2-oxoglutarate:O2 oxidoreductase (3-hydroxylating)
Systematic name: L-enduracididine,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Comments: Fe2+-dependent enzyme. The enzyme is involved in biosynthesis of the nonproteinogenic amino acid β-hydroxyenduracididine, a component of the mannopeptimycins (cyclic glycopeptide antibiotic), produced by Streptomyces hygroscopicus NRRL 30439.
Links to other databases: BRENDA, EXPASY, KEGG Metacyc, CAS registry number:
References:
1. Haltli, B., Tan, Y., Magarvey, N.A., Wagenaar, M., Yin, X., Greenstein, M., Hucul, J.A. and Zabriskie, T.M. Investigating β-hydroxyenduracididine formation in the biosynthesis of the mannopeptimycins. Chem. Biol. 12 (2005) 1163-1168. [PMID: 16298295]
2. Magarvey, N.A., Haltli, B., He, M., Greenstein, M. and Hucul, J.A. Biosynthetic pathway for mannopeptimycins, lipoglycopeptide antibiotics active against drug-resistant gram-positive pathogens. Antimicrob. Agents Chemother. 50 (2006) 2167-2177. [PMID: 16723579]