Reaction: UMP + 2-oxoglutarate + O2 = 5'-dehydrouridine + succinate + CO2 + phosphate
For diagram of reaction click here.
Glossary: 5'-dehydrouridine = uridine-5'-aldehyde
Other name(s): lipL (gene name)
Systematic name: UMP,2-oxoglutarate:oxygen oxidoreductase
Comments: The enzyme catalyses a net dephosphorylation and oxidation of UMP to generate 5'-dehydrouridine, the first intermediate in the biosynthesis of the unusual aminoribosyl moiety found in several C7-furanosyl nucleosides such as A-90289s, caprazamycins, liposidomycins, muraymycins and FR-900453. Requires Fe2+.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Yang, Z., Chi, X., Funabashi, M., Baba, S., Nonaka, K., Pahari, P., Unrine, J., Jacobsen, J.M., Elliott, G.I., Rohr, J. and Van Lanen, S.G. Characterization of LipL as a non-heme, Fe(II)-dependent α-ketoglutarate:UMP dioxygenase that generates uridine-5'-aldehyde during A-90289 biosynthesis. J. Biol. Chem. 286 (2011) 7885-7892. [PMID: 21216959]
2. Yang, Z., Unrine, J., Nonaka, K. and Van Lanen, S.G. Fe(II)-dependent, uridine-5'-monophosphate α-ketoglutarate dioxygenases in the synthesis of 5'-modified nucleosides. Methods Enzymol. 516 (2012) 153-168. [PMID: 23034228]