Reaction: L-glutamate + 2-oxoglutarate + O2 = (3R)-3-hydroxy-L-glutamate + succinate + CO2
Glossary: ibotenate = (S)-2-amino-2-(3-hydroxyisoxazol-5-yl)acetate
muscimol = 5-(aminomethyl)-1,2-oxazol-3-ol
Other name(s): iboH (gene name)
Systematic name: L-glutamate,2-oxoglutarate:oxygen oxidoreductase (3R-hydroxylating)
Comments: Requires Fe2+ and L-ascorbate. The enzyme, characterized from the basidiomycete mushroom Amanita muscaria, participates in the biosynthesis of the psychoactive compounds ibotenate and muscimol.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Muller, M. and Obermaier, S. Ibotenic acid biosynthesis in the fly agaric is initiated by glutamate hydroxylation. Angew. Chem. Int. Ed. Engl. 59 (2020) 12432-12435. [PMID: 32233056]