Reaction: benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
For diagram click here (another example).
Other name(s): benzoate hydroxylase; benzoate hydroxylase; benzoic hydroxylase; benzoate dioxygenase; benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, decarboxylating) [incorrect]
Systematic name: benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Comments: A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), and an iron-sulfur oxygenase. Requires Fe2+.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9059-18-1
References:
1. Yamaguchi, M. and Fujisawa, H. Characterization of NADH-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1. J. Biol. Chem. 253 (1978) 8848-8853. [PMID: 214433]
2. Yamaguchi, M. and Fujisawa, H. Purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1. J. Biol. Chem. 255 (1980) 5058-5063. [PMID: 7372624]
3. Yamaguchi, M. and Fujisawa, H. Subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from Pseudomonas arvilla C-1. J. Biol. Chem. 257 (1982) 12497-12502. [PMID: 7130163]