Reaction: salicylate + NADH + 2 H+ + O2 = catechol + NAD+ + H2O + CO2
For diagram of reaction click here or click here.
Other name(s): salicylate hydroxylase; salicylate 1-hydroxylase; salicylate monooxygenase; salicylate hydroxylase (decarboxylating)
Systematic name: salicylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)
Comments: A flavoprotein (FAD).
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9059-28-3
References:
1. Suzuki, K., Takemori, S. and Katagiri, M. Mechanism of the salicylate hydroxylase reaction. IV. Fluorimetric analysis of the complex formation. Biochim. Biophys. Acta 191 (1969) 77-85.
2. Takemori, S., Yasuda, H., Mihara, K., Suzuki, K. and Katagiri, M. Mechanism of the salicylate hydroxylase reaction. II. The enzyme-substrate complex. Biochim. Biophys. Acta 191 (1969) 58-68. [PMID: 4898626]
3. Takemori, S., Yasuda, H., Mihara, K., Suzuki, K. and Katagiri, M. Mechanism of the salicylate hydroxylase reaction. 3. Characterization and reactivity of chemically or photochemically reduced enzyme-flavin. Biochim. Biophys. Acta 191 (1969) 69-76. [PMID: 4309912]
4. Yamamoto, S., Katagiri, M., Maeno, H. and Hayaishi, O. Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide. J. Biol. Chem. 240 (1965) 3408-3413.