Reaction: 2,4-dichlorophenol + NADPH + H+ + O2 = 3,5-dichlorocatechol + NADP+ + H2O
Other name(s): 2,4-dichlorophenol hydroxylase; 2,4-dichlorophenol monooxygenase
Systematic name: 2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating)
Comments: A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, CAS registry number: 82047-82-3
References:
1. Beadle, C.A. and Smith, A.R.W. The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species. Eur. J. Biochem. 123 (1982) 323-332. [PMID: 7075592]
2. Perkins, E.J., Gordon, M.P., Caceres, O. and Lurquin, P.F. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J. Bacteriol. 172 (1990) 2351-2359. [PMID: 2185214]
3. Radjendirane, V., Bhat, M.A. and Vaidyanathan, C.S. Affinity purification and characterization of 2,4-dichlorophenol hydroxylase from Pseudomonas cepacia. Arch. Biochem. Biophys. 288 (1991) 169-176. [PMID: 1898014]
4. Ledger, T., Pieper, D.H. and Gonzalez, B. Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation. Appl. Environ. Microbiol. 72 (2006) 2783-2792. [PMID: 16597983]
5. Lu, Y., Yu, Y., Zhou, R., Sun, W., Dai, C., Wan, P., Zhang, L., Hao, D. and Ren, H. Cloning and characterisation of a novel 2,4-dichlorophenol hydroxylase from a metagenomic library derived from polychlorinated biphenyl-contaminated soil. Biotechnol. Lett. 33 (2011) 1159-1167. [PMID: 21293902]