Reaction: 2,4-dichlorophenol + NADPH + H+ + O2 = 3,5-dichlorocatechol + NADP+ + H2O
Other name(s): 2,4-dichlorophenol hydroxylase; 2,4-dichlorophenol monooxygenase
Systematic name: 2,4-dichlorophenol,NADPH:oxygen oxidoreductase (6-hydroxylating)
Comments: A flavoprotein (FAD). Also acts, more slowly, on 4-chlorophenol and 4-chloro-2-methylphenol; NADH can act instead of NADPH, but more slowly.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, CAS registry number: 82047-82-3
1. Beadle, C.A. and Smith, A.R.W. The purification and properties of 2,4-dichlorophenol hydroxylase from a strain of Acinetobacter species. Eur. J. Biochem. 123 (1982) 323-332. [PMID: 7075592]