IUBMB Enzyme Nomenclature


Accepted name: 1,3,7-trimethyluric acid 5-monooxygenase

Reaction: 1,3,7-trimethylurate + NADH + H+ + O2 = 1,3,7-trimethyl-5-hydroxyisourate + NAD+ + H2O

Glossary: isourate = 1,3,5,7-tetrahydropurine-2,6,8-trione

Other name(s): tmuM (gene name)

Systematic name: 1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)

Comments: The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC, FAD-dependent urate hydroxylase.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Mohanty, S.K., Yu, C.L., Das, S., Louie, T.M., Gakhar, L. and Subramanian, M. Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism. J. Bacteriol. 194 (2012) 3872-3882. [PMID: 22609920]

2. Summers, R.M., Mohanty, S.K., Gopishetty, S. and Subramanian, M. Genetic characterization of caffeine degradation by bacteria and its potential applications. Microb Biotechnol 8 (2015) 369-378. [PMID: 25678373]

[EC created 2016]

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