IUBMB Enzyme Nomenclature


Accepted name: 6-methylpretetramide 4-monooxygenase

Reaction: 6-methylpretetramide + NADPH + H+ + O2 = 4-hydroxy-6-methylpretetramide + NADP+ + H2O

For diagram of reaction click here.

Glossary: 6-methylpretetramide = 1,3,10,11,12-pentahydroxy-6-methyltetracene-2-carboxamide
4-hydroxy-6-methylpretetramide = 1,3,4,10,11,12-hexahydroxy-6-methyltetracene-2-carboxamide

Systematic name: 6-methylpretetramide,NADPH:oxygen oxidoreductase (4-hydroxylating)

Comments: The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. That bacterium possesses two enzymes that can catalyse the reaction - OxyE is the main isozyme, while OxyL has a lower activity. OxyL is bifunctional, and its main function is EC, 4-hydroxy-6-methylpretetramide 12a-monooxygenase. Contains FAD.

Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:


1. Zhang, W., Watanabe, K., Cai, X., Jung, M.E., Tang, Y. and Zhan, J. Identifying the minimal enzymes required for anhydrotetracycline biosynthesis. J. Am. Chem. Soc. 130 (2008) 6068-6069. [PMID: 18422316]

2. Wang, P., Zhang, W., Zhan, J. and Tang, Y. Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis. Chembiochem 10 (2009) 1544-1550. [PMID: 19472250]

[EC created 2016]

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