Reaction: 3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O
Other name(s): 3-hydroxybenzoate 6-hydroxylase; m-hydroxybenzoate 6-hydroxylase; 3-hydroxybenzoic acid-6-hydroxylase
Systematic name: 3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)
Comments: A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 51570-26-4
1. Groseclose, E.E. and Ribbons, D.W. 3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 55 (1973) 897-903. [PMID: 4357436]