IUBMB Enzyme Nomenclature


Accepted name: L-aspartate N-monooxygenase (nitrosuccinate-forming)

Reaction: L-aspartate + 3 NADPH + 3 H+ + 3 O2 = (2S)- 2-nitrobutanedioate + 3 NADP+ + 4 H2O
(1a) L-aspartate + NADPH + H+ + O2 = N-hydroxy-L-aspartate + NADP+ + H2O
(1b) N-hydroxy-L-aspartate + NADPH + H+ + O2 = N,N-dihydroxy-L-aspartate + NADP+ + H2O
(1c) N,N-dihydroxy-L-aspartate = (2S)-2-nitrosobutanedioate + H2O (spontaneous)
(1d) (2S)-2-nitrosobutanedioate + NADPH + H+ + O2 = (2S)-2-nitrobutanedioate + NADP+ + H2O

Glossary: 2-nitrobutanedioate = nitrosuccinate

Other name(s): creE (gene name)

Systematic name: L-aspartate,NADPH:oxygen oxidoreductase [(2S)-2-nitrobutanedioate-forming]

Comments: The enzyme, found in some Actinobacteria, is involved in a pathway that forms nitrite, which is subsequently used to generate a diazo group in some secondary metabolites. Requires an FAD cofactor.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Sugai, Y., Katsuyama, Y. and Ohnishi, Y. A nitrous acid biosynthetic pathway for diazo group formation in bacteria. Nat. Chem. Biol. 12 (2016) 73-75. [PMID: 26689788]

2. Hagihara, R., Katsuyama, Y., Sugai, Y., Onaka, H. and Ohnishi, Y. Novel desferrioxamine derivatives synthesized using the secondary metabolism-specific nitrous acid biosynthetic pathway in Streptomyces davawensis, J. Antibiot. (Tokyo) 71 (2018) 911-919. [PMID: 30120394]

[EC created 2021]

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