Reaction: 3-amino-4-hydroxybenzoate + NADPH + H+ + O2 = 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O
For diagram of reaction, click here
Other name(s): creL (gene name); ptmB3 (gene name); ptnB3 (gene name)
Systematic name: 3-amino-4-hydroxybenzoate,NADPH:oxygen oxidoreductase (2-hydroxylating)
Comments: Requires FAD. The CreL enzyme from the bacterium Streptomyces cremeus participates in the biosynthesis of cremeomycin. The PrmB3 and PtnB3 enzymes from Streptomyces platensis are involved in the biosynthesis of platensimycin and platencin, respectively.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Smanski, M.J., Yu, Z., Casper, J., Lin, S., Peterson, R.M., Chen, Y., Wendt-Pienkowski, E., Rajski, S.R. and Shen, B. Dedicated ent-kaurene and ent-atiserene synthases for platensimycin and platencin biosynthesis. Proc. Natl. Acad. Sci. USA 108 (2011) 13498-13503. [PMID: 21825154]
2. Waldman, A.J., Pechersky, Y., Wang, P., Wang, J.X. and Balskus, E.P. The cremeomycin biosynthetic gene cluster encodes a pathway for diazo formation. Chembiochem 16 (2015) 2172-2175. [PMID: 26278892]
3. Sugai, Y., Katsuyama, Y. and Ohnishi, Y. A nitrous acid biosynthetic pathway for diazo group formation in bacteria. Nat. Chem. Biol. 12 (2016) 73-75. [PMID: 26689788]
4. Dong, L.B., Rudolf, J.D., Kang, D., Wang, N., He, C.Q., Deng, Y., Huang, Y., Houk, K.N., Duan, Y. and Shen, B. Biosynthesis of thiocarboxylic acid-containing natural products. Nat. Commun. 9 (2018) 2362. [PMID: 29915173]