Reaction: putrescine + NADPH + H+ + O2 = N-hydroxyputrescine + NADP+ + H2O
For diagram of reaction click here
Other name(s): alcA (gene name); pubA (gene name); fbsI (gene name)
Systematic name: putrescine,NADPH:oxygen oxidoreductase (N-hydroxylating)
Comments: Contains FAD. The enzyme, characterized from multiple bacterial species, participates in the biosynthesis of assorted siderophores.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Kadi, N., Arbache, S., Song, L., Oves-Costales, D. and Challis, G.L. Identification of a gene cluster that directs putrebactin biosynthesis in Shewanella species: PubC catalyzes cyclodimerization of N-hydroxy-N-succinylputrescine. J. Am. Chem. Soc. 130 (2008) 10458-10459. [PMID: 18630910]
2. Li, B., Lowe-Power, T., Kurihara, S., Gonzales, S., Naidoo, J., MacMillan, J.B., Allen, C. and Michael, A.J. Functional identification of putrescine C- and N-hydroxylases. ACS Chem. Biol. 11 (2016) 2782-2789. [PMID: 27541336]
3. Lyons, N.S., Bogner, A.N., Tanner, J.J. and Sobrado, P. Kinetic and structural characterization of a flavin-dependent putrescine N-hydroxylase from Acinetobacter baumannii. Biochemistry 61 (2022) 2607-2620. [PMID: 36314559]