Reaction: 4-hydroxybenzoate + NAD(P)H + H+ + O2 = 3,4-dihydroxybenzoate + NAD(P)+ + H2O
Other name(s): 4-hydroxybenzoate 3-monooxygenase (reduced nicotinamide adenine dinucleotide (phosphate)); 4-hydroxybenzoate-3-hydroxylase; 4-hydroxybenzoate 3-hydroxylase; 4-hydroxybenzoate 3-monooxygenase [NAD(P)H2]
Systematic name: 4-hydroxybenzoate,NAD(P)H:oxygen oxidoreductase (3-hydroxylating)
Comments: A flavoprotein (FAD). The enzyme from Corynebacterium cyclohexanicum is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates (cf. EC 220.127.116.11 4-hydroxybenzoate 3-monooxygenase). It is less specific for NADPH2 than EC 18.104.22.168.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 95471-33-3
1. Fujii, T. and Kaneda, T. Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum. Eur. J. Biochem. 147 (1985) 97-104. [PMID: 3971979]
2. Seibold, B., Matthes, M., Eppink, M.H., Lingens, F., Van Berkel, W.J. and Muller, R. 4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity. Eur. J. Biochem. 239 (1996) 469-478. [PMID: 8706756]