Reaction: phenol + NADPH + H+ + O2 = catechol + NADP+ + H2O
For diagram of reaction click here.
Glossary: o-cresol = 2-cresol = 2-methylphenol
Other name(s): phenol hydroxylase; phenol o-hydroxylase
Systematic name: phenol,NADPH:oxygen oxidoreductase (2-hydroxylating)
Comments: A flavoprotein (FAD). The enzyme from the fungus Trichosporon cutaneum has a broad substrate specificity, and has been reported to catalyse the hydroxylation of a variety of substituted phenols, such as fluoro-, chloro-, amino- and methyl-phenols and also dihydroxybenzenes. cf. EC 22.214.171.124, phenol 2-monooxygenase (FADH2).
Links to other databases: BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37256-84-1
1. Nakagawa, H. and Takeda, Y. Phenol hydroxylase. Biochim. Biophys. Acta 62 (1962) 423-426. [PMID: 14478080]
2. Neujahr, H.Y. and Gaal, A. Phenol hydroxylase from yeast. Purification and properties of the enzyme from Trichosporon cutaneum, Eur. J. Biochem. 35 (1973) 386-400. [PMID: 4146224]
3. Neujahr, H.Y. and Gaal, A. Phenol hydroxylase from yeast. Sulfhydryl groups in phenol hydroxylase from Trichosporon cutaneum, Eur. J. Biochem. 58 (1975) 351-357. [PMID: 810352]