Reaction: 5,20-epoxytax-11-en-4α-ol + [reduced NADPH—hemoprotein reductase] + O2 = 5,20-epoxytax-11-ene-4α,9α-diol + [oxidized NADPH—hemoprotein reductase] + H2O
Other name(s): taxoid 9α hydroxylase; CYP725A22; T9αOH
Systematic name: 5,20-epoxytax-11-en-4α-ol,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (9α-hydroxylating)
Comments: The enzyme is active in the biosynthetic pathway of paclitaxel (Taxol) in Taxus species (yew)
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Zhang, Y., Wiese, L., Fang, H., Alseekh, S., Perez de Souza, L., Scossa, F., Molloy, J., Christmann, M. and Fernie, A.R. Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis. Mol. Plant 16 (2023) 1951-1961. [PMID: 37897038]