Reaction: 10-deacetyl-1-deoxybaccatin III + [reduced NADPH—hemoprotein reductase] + O2 = 10-deacetylbaccatin III + [oxidized NADPH—hemoprotein reductase] + H2O
For diagram of reaction click here
Other name(s): taxane 1β-hydroxylase; CYP725 A23-1; T1βOH
Systematic name: 10-deacetyl-1-deoxybaccatin III,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (1β-hydroxylating)
Comments: A cytochrome-P-450 (heme thiolate) enzyme involved in paclitaxel (taxol) biosynthesis. Isolated from the plant Taxus baccata (English yew).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Zhang, Y., Wiese, L., Fang, H., Alseekh, S., Perez de Souza, L., Scossa, F., Molloy, J., Christmann, M. and Fernie, A.R. Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis. Mol. Plant 16 (2023) 1951-1961. [PMID: 37897038]