Reaction: 3′-N-debenzoyl-2′-deoxytaxol + [reduced NADPH-hemoprotein reductase] + O2 = 3′-N-debenzoyltaxol + [oxidized NADPH-hemoprotein reductase] + H2O
For diagram of reaction click here
Other name(s): CYP73A171; T2'OH; TB506
Systematic name: 3′-N-debenzoyl-2′-deoxytaxol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (2′-hydroxylating)
Comments: A cytochrome-P-450 (heme thiolate) enzyme isolated from the plant Taxus × media. The penultimate step in paclitaxel (taxol) biosynthesis.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Sanchez-Munoz, R., Perez-Mata, E., Almagro, L., Cusido, R.M., Bonfill, M., Palazon, J. and Moyano, E. A novel hydroxylation step in the taxane biosynthetic pathway: a new approach to paclitaxel production by synthetic biology. Front Bioeng Biotechnol 8 (2020) 410. [PMID: 32528936]
2. Long, R.M. and Croteau, R. Preliminary assessment of the C13-side chain 2′-hydroxylase involved in taxol biosynthesis. Biochem. Biophys. Res. Commun. 338 (2005) 410-417. [PMID: 16137660]