IUBMB Enzyme Nomenclature


Accepted name: (S)-limonene 7-monooxygenase

Reaction: (S)-limonene + [reduced NADPH —hemoprotein reductase] + O2 = (–)-perillyl alcohol + [oxidized NADPH —hemoprotein reductase] + H2O

For diagram of reaction click here

Glossary: limonene = a monoterpenoid
(S)-limonene = (–)-limonene

Other name(s): (–)-limonene 7-monooxygenase; (–)-limonene hydroxylase; (–)-limonene monooxygenase; (–)-limonene,NADPH:oxygen oxidoreductase (7-hydroxylating)

Systematic name: (S)-limonene,[reduced NADPH —hemoprotein reductase]:oxygen oxidoreductase (7-hydroxylating)

Comments: A cytochrome P-450 (heme thiolate) enzyme. The enzyme, characterized from the plant Perilla frutescens, participates in the biosynthesis of perillyl aldehyde, the major constituent of the essential oil that accumulates in the glandular trichomes of this plant. Some forms of the enzyme also catalyse the oxidation of (–)-perillyl alcohol to (–)-perillyl aldehyde.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Karp, F., Mihaliak, C.A., Harris, J.L. and Croteau, R. Monoterpene biosynthesis: specificity of the hydroxylations of (–)-limonene by enzyme preparations from peppermint (Mentha piperita), spearmint (Mentha spicata), and perilla (Perilla frutescens) leaves. Arch. Biochem. Biophys. 276 (1990) 219-226. [PMID: 2297225]

2. Mau, C.J., Karp, F., Ito, M., Honda, G. and Croteau, R.B. A candidate cDNA clone for (–)-limonene-7-hydroxylase from Perilla frutescens. Phytochemistry 71 (2010) 373-379. [PMID: 20079506]

3. Fujiwara, Y. and Ito, M. Molecular cloning and characterization of a Perilla frutescens cytochrome P450 enzyme that catalyzes the later steps of perillaldehyde biosynthesis. Phytochemistry 134 (2017) 26-37. [PMID: 27890582]

[EC created 1992 as EC, modified 2003, transferred 2017 to EC]

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