Reaction: phenylacetate + [reduced NADPH —hemoprotein reductase] + O2 = (2-hydroxyphenyl)acetate + [oxidized NADPH —hemoprotein reductase] + H2O
Other name(s): CYP504; phaA (gene name)
Systematic name: phenylacetate,[reduced NADPH —hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Comments: This cytochrome P-450 (heme-thiolate) enzyme, found in Aspergillus nidulans, is involved in the degradation of phenylacetate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Mingot, J.M., Penalva, M.A. and Fernandez-Canon, J.M. Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction. J. Biol. Chem. 274 (1999) 14545-14550. [PMID: 10329644]
2. Rodriguez-Saiz, M., Barredo, J.L., Moreno, M.A., Fernandez-Canon, J.M., Penalva, M.A. and Diez, B. Reduced function of a phenylacetate-oxidizing cytochrome P450 caused strong genetic improvement in early phylogeny of penicillin-producing strains. J. Bacteriol. 183 (2001) 5465-5471. [PMID: 11544206]