Reaction: β-amyrin + [reduced NADPHhemoprotein reductase] + O2 = maniladiol + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Glossary: cochalic acid = 3β,16β-dihydroxyolean-12-en-28-oic acid
maniladiol = 16β-hydroxy-β-amyrin = olean-12-ene-3β,16β-diol
Other name(s): CYP716A141
Systematic name: β-amyrin,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (maniladiol forming)
Comments: A cytochrome P-450 (heme-thiolate) protein isolated from the plant Platycodon grandiflorus (baloon flower). The enzyme is also able to oxidize oleanolic acid to cochalic acid.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Tamura, K., Teranishi, Y., Ueda, S., Suzuki, H., Kawano, N., Yoshimatsu, K., Saito, K., Kawahara, N., Muranaka, T. and Seki, H. Cytochrome P450 monooxygenase CYP716A141 is a unique β-amyrin C-16β oxidase Involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus. Plant Cell Physiol 58 (2017) 874-884. [PMID: 28371833]