Reaction: an isoflavone + [reduced NADPHhemoprotein reductase] + O2 = a 2'-hydroxyisoflavone + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Other name(s): isoflavone 2'-monooxygenase; CYP81E1; CYP Ge-3
Systematic name: isoflavone,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Comments: A cytochrome P-450 (heme-thiolate) protein. Acts on daidzein, formononetin and genistein. EC 220.127.116.11, 4'-methoxyisoflavone 2'-hydroxylase, has the same reaction but is more specific as it requires a 4'-methoxyisoflavone.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Akashi, T., Aoki, T. and Ayabe, S.-I. CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase. Biochem. Biophys. Res. Commun. 251 (1998) 67-70. [PMID: 9790908]