Reaction: protopine + [reduced NADPHhemoprotein reductase] + O2 = 6-hydroxyprotopine + [oxidized NADPHhemoprotein reductase] + H2O
For diagram of reaction click here.
Other name(s): protopine 6-hydroxylase; CYP82N2 (gene name)
Systematic name: protopine,[reduced NADPHhemoprotein reductase]:oxygen oxidoreductase (6-hydroxylating)
Comments: A cytochrome P-450 (heme-thiolate) protein involved in benzophenanthridine alkaloid synthesis in higher plants.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number:
1. Tanahashi, T. and Zenk, M.H. Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis. Phytochemistry 29 (1990) 1113-1122.
2. Takemura, T., Ikezawa, N., Iwasa, K. and Sato, F. Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells. Phytochemistry 91 (2013) 100-108. [PMID: 22421633]