IUBMB Enzyme Nomenclature


Accepted name: chloroacetanilide N-alkylformylase

Reaction: butachlor + 2 reduced ferredoxin [iron-sulfur] cluster + O2 = 2-chloro-N-(2,6-diethylphenyl)acetamide + butyl formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O

Glossary: butachlor = N-(butoxymethyl)-2-chloro-N-(2,6-diethylphenyl)acetamide
acetochlor = N-(ethoxymethyl)-2-chloro-N-(2-ethyl,6-methylphenyl)acetamide
alachlor = N-(methoxymethyl)-2-chloro-N-(2,6-diethylphenyl)acetamide

Other name(s): cndA (gene name)

Systematic name: butachlor,ferredoxin:oxygen oxidoreductase (butyl formate-releasing)

Comments: The enzyme, characterized from the bacterium Sphingomonas sp. DC-6, initiates the degradation of several chloroacetanilide herbicides, including alachlor, acetochlor, and butachlor. The enzyme is a Rieske non-heme iron oxygenase, and requires a ferredoxin and EC, ferredoxin-NAD+ reductase, for activity.

Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:


1. Chen, Q., Wang, C.H., Deng, S.K., Wu, Y.D., Li, Y., Yao, L., Jiang, J.D., Yan, X., He, J. and Li, S.P. Novel three-component Rieske non-heme iron oxygenase system catalyzing the N-dealkylation of chloroacetanilide herbicides in sphingomonads DC-6 and DC-2. Appl. Environ. Microbiol. 80 (2014) 5078-5085. [PMID: 24928877]

[EC created 2017]

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