Reaction: cyclohexane-1-carboxylate + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = trans-4-hydroxycyclohexane-1-carboxylate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
Glossary: trans-4-hydroxycyclohexane-1-carboxylate = trans-4-hydroxycyclohexanecarboxylate
Other name(s): chcAa (gene name)
Systematic name: cyclohexane-1-carboxylate,ferredoxin:oxygen oxidoreductase (4-trans-hydroxylating)
Comments: The enzyme, characterized from the bacterium Corynebacterium cyclohexanicum, is a cytochrome P-450 enzyme that participates in the degradation pathway of cyclohexane-1-carboxylate. The initial source of the electrons is NADH, which transfers the electrons to a specific [2Fe-2S] ferredoxin via a dedicated EC 1.18.1.3, ferredoxin—NAD+ reductase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Yamamoto, T., Hasegawa, Y., Lau, P.CK. and Iwaki, H. Identification and characterization of a chc gene cluster responsible for the aromatization pathway of cyclohexanecarboxylate degradation in Sinomonas cyclohexanicum ATCC 51369. J. Biosci. Bioeng. 132 (2021) 621-629. [PMID: 34583900]