Reaction: L-tyrosine + nitric oxide + O2 + reduced ferredoxin + H+ = 3-nitro-L-tyrosine + H2O + oxidized ferredoxin
Other name(s): rufO (gene name); 3-nitro-tyrosine synthase
Systematic name: L-tyrosine,nitric oxide, reduced ferredoxin:oxygen oxidoreductase (3-nitro-L-tyrosine-forming)
Comments: A cytochrome P-450 enzyme characterized from the bacterium Streptomyces atratus. The enzyme participates in biosynthesis of rufomycins, circular heptapeptides with anti-mycobacterial activity. The enzyme requires ferredoxin as its electron donor and catalyses a reaction in which nitric oxide is oxidized to a nitro group and inserted into L-tyrosine at the 3 position (not necessarily in this order).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Tomita, H., Katsuyama, Y., Minami, H. and Ohnishi, Y. Identification and characterization of a bacterial cytochrome P450 monooxygenase catalyzing the 3-nitration of tyrosine in rufomycin biosynthesis. J. Biol. Chem. 292 (2017) 15859-15869. [PMID: 28774961]