Reaction: nocardicin C + 2 O2 + 4 reduced ferredoxin + 4 H+ = nocardicin A + 3 H2O + 4 oxidized ferredoxin (overall reaction)
(1a) nocardicin C + O2 + 2 reduced ferredoxin + 2 H+ = 2'-N-hydroxynocardicin C + H2O + 2 oxidized ferredoxin
(1b) 2'-N-hydroxynocardicin C + O2 + 2 reduced ferredoxin + 2 H+ = 2'-N,N-dihydroxynocardicin C + 2 oxidized ferredoxin
(1c) 2'-N,N-dihydroxynocardicin C = nocardicin A + H2O
For diagram of reaction click here
Glossary: nocardicin C = (2R)-2-amino-4-{4-[(R)-amino({[(3S)-1-[(R)-carboxy(4-hydroxyphenyl)methyl]-2-oxoazetidin-3-yl]carbamoyl})methyl]phenoxy}butanoate
nocardicin A = (2R)-2-amino-4-{4-[(1Z)-{[(3S)-1-[(R)-carboxy(4-hydroxyphenyl)methyl]-2-oxoazetidin-3-yl]carbamoyl}(hydroxyimino)methyl]phenoxy}butanoate
Other name(s): nocL (gene name)
Systematic name: nocardicin C,reduced ferredoxin:O2 oxidoreductase (nocardicin A-forming)
Comments: This cytochrome P-450 enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Kelly, W.L. and Townsend, C.A. Role of the cytochrome P450 NocL in nocardicin A biosynthesis. J. Am. Chem. Soc. 124 (2002) 8186-8187. [PMID: 12105888]
2. Kelly, W.L. and Townsend, C.A. Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis. J. Bacteriol. 187 (2005) 739-746. [PMID: 15629944]