Reaction: 7-chloro-L-tryptophan + FADH2 + chloride + O2 + H+ = 6,7-dichloro-L-tryptophan + FAD + 2 H2O
For diagram of reaction click here.
Other name(s): ktzR (gene name)
Systematic name: 7-chloro-L-tryptophan:FADH2 oxidoreductase (6-halogenating)
Comments: An FAD-dependent halogenase. The enzyme, characterized from the bacterium Kutzneria sp. 744, works in tandem with EC 220.127.116.11, tryptophan 7-halogenase, (ktzQ) to generate 6,7-dichloro-L-tryptophan, which is incorporated as a pyrroloindoline in the kutznerides family of natural products. It has a 120-fold preference for 7-chloro-L-tryptophan over L-tryptophan as substrate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Heemstra, J.R., Jr. and Walsh, C.T. Tandem action of the O2- and FADH2-dependent halogenases KtzQ and KtzR produce 6,7-dichlorotryptophan for kutzneride assembly. J. Am. Chem. Soc. 130 (2008) 14024-14025. [PMID: 18828589]