Reaction: 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + a reduced acceptor + O2 = 3-demethylubiquinol + acceptor + H2O
For diagram of reaction click here
Glossary: 3-demethylubiquinol = 3-methoxy-6-methyl-5-(all trans-polyprenyl)benzene-1,2,4-triol
Other name(s): 6-methoxy-3-methyl-2-(all-trans-polyprenyl)-1,4-benzoquinol 5-hydroxylase; COQ7 (gene name); clk-1 (gene name); ubiF (gene name); 6-methoxy-3-methyl-2-(all-trans-polyprenyl)-1,4-benzoquinol,acceptor:oxygen oxidoreductase (5-hydroxylating)
Systematic name: 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol,acceptor:oxygen oxidoreductase (5-hydroxylating)
Comments: This prokaryotic enzyme catalyses the last hydroxylation reaction during the biosynthesis of ubiquinone. The eukaryotic enzyme is classified under EC 1.14.13.253, 3-demethoxyubiquinone 3-hydroxylase (NADH).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number:
References:
1. Kwon, O., Kotsakis, A. and Meganathan, R. Ubiquinone (coenzyme Q) biosynthesis in Escherichia coli: identification of the ubiF gene. FEMS Microbiol. Lett. 186 (2000) 157-161. [PMID: 10802164]