Reaction: 6-hydroxynicotinate + H2O + O2 = 2,6-dihydroxynicotinate + H2O2
Other name(s): 6-hydroxynicotinic acid hydroxylase; 6-hydroxynicotinic acid dehydrogenase; 6-hydroxynicotinate hydroxylase; 6-hydroxynicotinate:O2 oxidoreductase
Systematic name: 6-hydroxynicotinate:oxygen oxidoreductase
Comments: Contains [2Fe-2S] iron-sulfur centres, FAD and molybdenum. It also has a catalytically essential, labile selenium that can be removed by reaction with cyanide. In Bacillus niacini, this enzyme is required for growth on nicotinic acid.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 122191-32-6
1. Nagel, M. and Andreesen, J.R. Molybdenum-dependent degradation of nicotinic acid by Bacillus sp. DSM 2923. FEMS Microbiol. Lett. 59 (1989) 147-152.
2. Nagel, M. and Andreesen, J.R. Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini. Arch. Microbiol. 154 (1990) 605-613.