Reaction: D-glyceraldehyde 3-phosphate + arsenate + NAD+ = 1-arsono-3-phospho-D-glycerate + NADH + H+
Glossary: 1-arsono-3-phosphoglycerate = [(2R)-2-hydroxy-3-phosphopropanoyl]oxyarsonate
Systematic name: D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (arsenate-transferring)
Comments: The enzyme, discovered in bacteria, is very similar to EC 220.127.116.11, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). However, the gene encoding it is located in arsenic resistance islands in the chromosome, next to a gene (arsJ) that encodes a transporter that removes the product, 1-arseno-3-phosphoglycerate, from the cell. Together the two proteins form an arsenic detoxification system.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Chen, J., Yoshinaga, M., Garbinski, L.D. and Rosen, B.P. Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance. Mol. Microbiol. 100 (2016) 945-953. [PMID: 26991003]
2. Wu, S., Wang, L., Gan, R., Tong, T., Bian, H., Li, Z., Du, S., Deng, Z. and Chen, S. Signature arsenic detoxification pathways in Halomonas sp. strain GFAJ-1. mBio 9 (2018) . [PMID: 29717010]