Reaction: (2R)-2-hydroxy-3-oxopropanoyl-[acyl-carrier-protein] + H2O + acceptor = a (2R)-2-hydroxymalonyl-[acyl-carrier-protein] + reduced acceptor
Other name(s): fkbI (gene name); asm15 (gene name); zmaE (gene name)
Systematic name: a (2R)-2-hydroxy-3-oxopropanoyl-[acp]:acceptor oxidoreductase (3-hydroxylating)
Comments: Contains an FAD cofactor. The enzyme participates in the biosynthesis of 2-hydroxymalonate and 2-methoxymalonate, unusual polyketide synthase extender units used in the biosynthesis of some polyketide products, such as ascomycin (FK520), tacrolimus (FK506) and the ansamitocins.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Wu, K., Chung, L., Revill, W.P., Katz, L. and Reeves, C.D. The FK520 gene cluster of Streptomyces hygroscopicus var. ascomyceticus (ATCC 14891) contains genes for biosynthesis of unusual polyketide extender units. Gene 251 (2000) 81-90. [PMID: 10863099]
2. Kato, Y., Bai, L., Xue, Q., Revill, W.P., Yu, T.W. and Floss, H.G. Functional expression of genes involved in the biosynthesis of the novel polyketide chain extension unit, methoxymalonyl-acyl carrier protein, and engineered biosynthesis of 2-desmethyl-2-methoxy-6-deoxyerythronolide B. J. Am. Chem. Soc. 124 (2002) 5268-5269. [PMID: 11996558]
3. Watanabe, K., Khosla, C., Stroud, R.M. and Tsai, S.C. Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis. J. Mol. Biol. 334 (2003) 435-444. [PMID: 14623185]
4. Chan, Y.A., Boyne, M.T., 2nd, Podevels, A.M., Klimowicz, A.K., Handelsman, J., Kelleher, N.L. and Thomas, M.G. Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units. Proc. Natl. Acad. Sci. USA 103 (2006) 14349-14354. [PMID: 16983083]