Reaction: an aldehyde + acceptor + H2O = a carboxylate + reduced acceptor
Other name(s): aldehyde:(acceptor) oxidoreductase
Systematic name: aldehyde:acceptor oxidoreductase
Comments: Contains bis(tungstenpterin) cofactor, FAD, and iron-sulfur clusters. While the physiological electron acceptor is thought to be ferredoxin, the enzyme from Aromatoleum aromaticum can use benzyl viologen, NAD+, and molecular hydrogen. Unlike some other aldehyde oxidorductases, in the presence of strong reductants this enzyme is able to catalyse the reverse reaction, reducing non-activated acids to aldehydes. cf. EC 1.2.7.5, aldehyde ferredoxin oxidoreductase. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 125008-36-8
References:
1. White, H., Strobl, G., Feicht, R. and Simon, H. Carboxylic acid reductase: a new tungsten enzyme catalyses the reduction of non-activated carboxylic acids to aldehydes. Eur. J. Biochem. 184 (1989) 89-96. [PMID: 2550230]
2. Debnar-Daumler, C., Seubert, A., Schmitt, G. and Heider, J. Simultaneous involvement of a tungsten-containing aldehyde:ferredoxin oxidoreductase and a phenylacetaldehyde dehydrogenase in anaerobic phenylalanine metabolism. J. Bacteriol. 196 (2014) 483-492. [PMID: 24214948]
3. Schmitt, G., Arndt, F., Kahnt, J. and Heider, J. Adaptations to a loss-of-function mutation in the betaproteobacterium Aromatoleum aromaticum: recruitment of alternative enzymes for anaerobic phenylalanine degradation. J. Bacteriol. 199 (2017) . [PMID: 28784814]
4. Arndt, F., Schmitt, G., Winiarska, A., Saft, M., Seubert, A., Kahnt, J. and Heider, J. Characterization of an aldehyde oxidoreductase from the mesophilic bacterium Aromatoleum aromaticum EbN1, a member of a new subfamily of tungsten-containing enzymes. Front Microbiol. 10 (2019) 71. [PMID: 30766522]
5. Winiarska, A., Hege, D., Gemmecker, Y., Krysciak-Czerwenka, J., Seubert, A., Heider, J. and Szaleniec, M. Tungsten enzyme using hydrogen as an electron donor to reduce carboxylic acids and NAD+. ACS Catal. 12 (2022) 8707-8717. [PMID: 35874620]
6. Rasbach, D.A., Basarab, R.M., Friedlander, M. and Harrison, T.S. Thallium-technetium subtraction scanning for preoperative localization of adenomas of the parathyroid gland. Surg Gynecol Obstet 163 (1986) 82-84. [PMID: 3726735]