Reaction: 2 columbamine + O2 = 2 berberine + 2 H2O
For diagram click here.
Other name(s): berberine synthase
Systematic name: columbamine:oxygen oxidoreductase (cyclizing)
Comments: An iron protein. Oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 95329-18-3
References:
1. Rueffer, M. and Zenk, M.H. Berberine synthesis, the methylenedioxy group forming enzyme in berberine synthesis. Tetrahedron Lett. 26 (1985) 201-202.