Reaction: cyclohexane-1-carbonyl-CoA + NADP+ = cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+
Other name(s): 1-cyclohexenylcarbonyl-CoA reductase (ambiguous); chcA (gene name)
Systematic name: cyclohexane-1-carbonyl-CoA:NADP+ 1-oxidoreductase
Comments: The enzyme, characterized from the bacterium Streptomyces collinus, is involved in a pathway that transforms shikimate to cyclohexane-1-carbonyl-CoA by a series of dehydration and double-bond reduction steps. Most of the steps in this process occur with the carboxylic acid activated as a coenzyme A thioester. The enzyme catalyses three steps in this pathway, also acting on (3R,4R)-3,4-dihydroxycyclohexa-1,5-diene-1-carbonyl-CoA and (5S)-5-hydroxycyclohex-1-ene-1-carbonyl-CoA.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
1. Reynolds, K.A., Wang, P., Fox, K.M., Speedie, M.K., Lam, Y. and Floss, H.G. Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus. J. Bacteriol. 174 (1992) 3850-3854. [PMID: 1597409]
2. Wang, P., Denoya, C.D., Morgenstern, M.R., Skinner, D.D., Wallace, K.K., Digate, R., Patton, S., Banavali, N., Schuler, G., Speedie, M.K. and Reynolds, K.A. Cloning and characterization of the gene encoding 1-cyclohexenylcarbonyl coenzyme A reductase from Streptomyces collinus. J. Bacteriol. 178 (1996) 6873-6881. [PMID: 8955309]