Reaction: (1) 4-ethylphenol + NAD+ = 4-vinylphenol + NADH + H+
(2) 4-ethylcatechol + NAD+ = 4-vinylcatechol + NADH + H+
(3) 4-ethylguaiacol + NAD+ = 4-vinylguaiacol + NADH + H+
Other Names: vprA (gene name)
Systematic name: 4-ethylphenol:NAD+ 7-oxidoreductase
Comments: Requires FAD. The enzyme, characterized from the lactic acid bacterium Lactiplantibacillus plantarum and the yeast Brettanomyces bruxellensis, is involved in the production of ethylphenols during the degradation of hydroxycinnamic acids.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. Tchobanov, I., Gal, L., Guilloux-Benatier, M., Remize, F., Nardi, T., Guzzo, J., Serpaggi, V. and Alexandre, H. Partial vinylphenol reductase purification and characterization from Brettanomyces bruxellensis. FEMS Microbiol. Lett. 284 (2008) 213-217. [PMID: 18576949]
2. Granato, T.M., Romano, D., Vigentini, I., Foschino, R.C., Monti, D., Mamone, G., Ferranti, P., Nitride, C., Iametti, S., Bonomi, F. and Molinari, F. New insights on the features of the vinyl phenol reductase from the wine-spoilage yeast Dekkera/Brettanomyces bruxellensis. Ann Microbiol 65 (2015) 321-329.
3. Romano, D., Valdetara, F., Zambelli, P., Galafassi, S., De Vitis, V., Molinari, F., Compagno, C., Foschino, R. and Vigentini, I. Cloning the putative gene of vinyl phenol reductase of Dekkera bruxellensis in Saccharomyces cerevisiae. Food Microbiol 63 (2017) 92-100. [PMID: 28040186]
4. Santamaria, L., Reveron, I., Lopez de Felipe, F., de Las Rivas, B. and Munoz, R. Unravelling the reduction pathway as an alternative metabolic route to hydroxycinnamate decarboxylation in Lactobacillus plantarum. Appl. Environ. Microbiol. 84 (2018) e01123-18. [PMID: 29776925]