Reaction: butanoate + NAD+ = but-2-enoate + NADH + H+
Other name(s): enoate reductase
Systematic name: butanoate:NAD+ Δ2-oxidoreductase
Comments: An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl α,β-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 70712-51-5
References:
1. Tischer, W., Bader, J. and Simon, H. Purification and some properties of a hitherto-unknown enzyme reducing the carbon-carbon double bond of α,β-unsaturated carboxylate anions. Eur. J. Biochem. 97 (1979) 103-112. [PMID: 477658]