Reaction: 23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
Other name(s): NAD(P)H: cucurbitacin B Δ23-oxidoreductase
Systematic name: 23,24-dihydrocucurbitacin:NAD(P)+ Δ23-oxidoreductase
Comments: Requires Mn2+. Fe2+ or Zn2+ can replace Mn2+ to some extent.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 37256-38-5
References:
1. Schabort, J.C. and Potgieter, D.J.J. Cucurbitacin B Δ23-reductase from Cucurbita maxima. II. Cofactor requirements, enzyme kinetics, substrate specificity and other characteristics. Biochim. Biophys. Acta 151 (1968) 47-53. [PMID: 4384331]
2. Schabort, J.C., Potgieter, D.J.J. and de Villiers, V. Cucurbitacin B Δ23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification. Biochim. Biophys. Acta 151 (1968) 33-46. [PMID: 5640163]