Reaction: (S)-tetrahydroberberine + 2 O2 = berberine + 2 H2O2
For diagram click here or here.
Other name(s): (S)-THB oxidase
Systematic name: (S)-tetrahydroberberine:oxygen oxidoreductase
Comments: The enzyme from Berberis sp. (previously listed as EC 18.104.22.168) is a flavoprotein; that from Coptis japonica is not. (R)-Tetrahydroberberines are not oxidized.
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 114705-00-9
1. Amann, M., Nagakura, N. and Zenk, M.H. (S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis. Tetrahedron Lett. 25 (1984) 953-954.
2. Okada, N., Shinmyo, A., Okada, H. and Yamada, Y. Purification and characterization of (S)-tetrahydroberberine oxidase from cultured Coptis japonica cells. Phytochemistry 27 (1988) 979-982.