Reaction: 2 L-glutamate + NAD+ = L-glutamine + 2-oxoglutarate + NADH + H+
(1a) L-glutamate + NH3 = L-glutamine + H2O
(1b) L-glutamate + NAD+ + H2O = NH3 + 2-oxoglutarate + NADH + H+
For diagram of reaction click here.
Other name(s): glutamate (reduced nicotinamide adenine dinucleotide) synthase; NADH: GOGAT; L-glutamate synthase (NADH); L-glutamate synthetase; NADH-glutamate synthase; NADH-dependent glutamate synthase
Systematic name: L-glutamate:NAD+ oxidoreductase (transaminating)
Comments: A flavoprotein (FMN). The reaction takes place in the direction of L-glutamate production. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 126.96.36.199, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 188.8.131.52, glutamate dehydrogenase).
Links to other databases: BRENDA, EXPASY, ExplorEnz, IUBMB, KEGG, MetaCyc, CAS registry number: 65589-88-0
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